李庆刚,博士生导师,天津市青年拔尖人才,中国科学院青年创新促进会会员;伟德BETVLCTOR特聘教授;
主要研究方向为食品功能因子基因工程菌种的构建、智能高通量进化筛选、以及发酵工艺优化;主持2项省部级人才项目,5项国家及省部级科研项目,核心参与多项企业项目;发表SCI论文15篇,申请专利9项(其中4项申请国际专利),授权4项;相关菌种成功推广到企业,为企业新增加利润超过2亿元。
主持项目:
序号 |
起止时间 |
项目(课题)名称 |
经费来源及额度 |
担任角色 |
1 |
2017.1-2019.12 |
天津市人才发展特殊支持计划“青年拔尖人才” |
天津市(人才项目) 100万 |
主持人 |
2 |
2016.1-2019.12 |
中科院青年创新促进会会员资助项目 |
中国科学院(人才项目) 70万 |
主持人 |
3 |
2014.1-2016.12 |
体内以DNA为中心的互作蛋白的鉴定与定量方法学研究 |
国家自然基金青年基金 22万 |
主持人 |
4 |
2019.1-2022.12 |
以异亮氨酰-tRNA合成酶为例构建一类新型氨基酸生物传感器 |
国家自然基金面上项目 59万 |
主持人 |
5 |
2012.11-2015.12 |
赖氨酸生产菌株的基因组理性设计和构建 |
863项目子课题 255万 |
主持人 |
6 |
2013.1-2015.12 |
氨基酸产业科技创新中心建设 |
中科院项目子课题 70万 |
主持人 |
7 |
2015.1-2015-12 |
氨基酸产业知识产权发展战略研究与应用示范 |
中科院STS项目子课题 10万 |
主持人 |
发表论文
1) Sun, Xue†; Li, Qinggang†; Wang, Yu†; Zhou, Wenjuan; Guo, Yanmei; Chen, Jiuzhou; Zheng, Ping; Sun, Jibin; Ma, Yanhe; Isoleucyl-tRNA synthetase mutant based whole-cell biosensor for high-throughput selection of isoleucine overproducers., Biosensors and Bioelectronics, 2021, 172: 112783. († 共同第一作者,员工在前).
2) Taiwo Dele-Osibanjo†, Li Qinggang†, Zhang Xiaoli, Guo Xuan, Feng Jinhui, Liu Jiao, Sun Xue, Wang Xiaowei, Zhou Wenjuan, Zheng Ping, Sun Jibin, MaYanhe. Growth-coupled evolution of phosphoketolase to improve L-glutamate production by Corynebacterium glutamicum. Applied Microbiology and Biotechnology. 2019. 103. 8413–8425. († 共同第一作者,员工在前).
3) Wang Xiaowei†, Li Qinggang†, Sun Cunmin, Cai Zhen, Zheng Xiaomei, Guo Xuan, Ni Xiaomeng, Zhou Wenjuan, Guo Yanmei, Zheng Ping, Chen Ning, Sun Jibin, Li Yin and Ma Yanhe. GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli. Microbial Cell Factories. 2019, 18:106. († 共同第一作者,员工在前).
4) Tuyishime Philibert†, Wang Yu†, Fan Liwen, Zhang Qiongqiong, Li Qingqang, Zheng Ping, Sun Jibin, Ma Yanhe. Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production. Metabolic engineering. 2018. 49: 220–231. (†共同第一作者).
5) Fan Liwen†, Wang Yu†, Tuyishime Philibert, Gao Ning, Li Qinggang, Zheng Ping, Sun Jibin, Ma Yanhe. Engineering artificial fusion proteins for enhanced methanol bioconversion. Chembiochem. 2018. 19: 2465–2471. (†共同第一作者).
6) Wang Xiaolu†, Wang Yu†, Liu Jiao, Li Qinggang, Zhang Zhidan, Zheng Ping, Lu Fuping, Sun Jibin. Biological conversion of methanol by evolved Escherichia coli carrying a linear methanol assimilation pathway. Bioresources and Bioprocessing. 2017, 4: 1–6. (†共同第一作者).
7) Song Yafeng, Nikoloff Jonas M, Fu Gang, Chen Jingqi, Li Qinggang, Xie Nengzhong, Zheng Ping, Sun Jibin, Zhang Dawei. Promoter screening from Bacillus subtilis in various conditions hunting for synthetic biology and industrial applications. Plos One. 2016. 11(7).
8) Wang Yan†, Li Qinggang†, Zheng Ping, Guo Yanmei, Wang Lixian, Zhang Tongcun, Sun Jibin, Ma Yanhe. Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method. Journal of Industrial Microbiology & Biotechnology. 2016. 43: 1227–1235. (†共同第一作者,员工在前).
9) Liu Ya-nan†, Li Qinggang†, Zheng Ping, Zhang Zhidan, Liu Yongfei, Sun Cunmin, Cao Guoqiang, Zhou Wenjuan, Wang Xiaowei, Zhang Dawei, Zhang Tongcun, Sun Jibin, Ma Yanhe. Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories. 2015. 14: 121. († 共同第一作者,员工在前).
10) Tang Hongzhi†, Yu Hao†, Li Qinggang†, Wang Xiaoyu, Gai Zhonghui, Yin Guangbo, Su Fei, Tao Fei, Ma Cuiqing, Xu Ping. Genome sequence of Pseudomonas putida strain B6-2, a superdegrader of polycyclic aromatic hydrocarbons and dioxin-like compounds. Journal of Bacteriology. 2011. 193: 6789–6790. (†共同第一作者).
11) Li Qinggang, Wang Xiaoyu, Yin Guangbo, Gai Zhonghui, Tang Hongzhi, Ma Cuiqing, Deng Zixin, Xu Ping. New metabolites in dibenzofuran cometabolic degradation by a biphenyl-cultivated Pseudomonas putida strain B6-2. Environmental Science and Technology. 2009. 43: 8635–8642.
12) Li, Li, Li Qinggang, Li Fuli, Shi Quan, Yu Bo, Liu Fengrui, Xu Ping. Degradation of carbazole and its derivatives by a Pseudomonas sp. Applied Microbiology and Biotechnology. 2006. 73, 941–948.
13) Wang Limin, Zhao Bo, Liu Bo, Yu Bo, Ma Cuiqing, Su Fei, Hua Dongliang, Li Qinggang, Ma Yanhe, Xu Ping. Efficient production of l-lactic acid from corncob molasses, a waste by-product in xylitol production, by a newly isolated xylose utilizing Bacillus sp. strain. Bioresource Technology. 2010. 101, 7908–7915.
14) Wang Limin, Zhao Bo, Li Fengsong, Xu Ke, Ma Cuiqing, Tao Fei, Li Qinggang and Xu Ping. Highly efficient production of D-lactate by Sporolactobacillus sp. CASD with simultaneous enzymatic hydrolysis of peanut meal. Applied Microbiology and Biotechnology. 2010. 89, 1009–1017.
15) Wang Limin, Zhao Bo, Liu Bo, Yang Chunyu, Yu Bo, Li Qinggang, Ma Cuiqing, Xu Ping, Ma Yanhe. Efficient production of l-lactic acid from cassava powder by Lactobacillus rhamnosus. Bioresource Technology. 2010. 101, 7895–7901.
(三)、申请专利
1) 一种生产2’-岩藻糖基乳糖的大肠杆菌及其应用.申请号:202110134041.X.申请日:2021.2.1.中国申请.
2) MviN蛋白突变体、含有该突变体的表达载体和宿主细胞及其应用.申请号:201910787948.9.申请日:2019.08.26.中国申请.国际申请.中国已授权.
3) 赖氨酸生产菌株的构建方法和应用.申请号:201910393041.4申请日:2019.5.30.中国申请.
4) 活性提高的磷酸转酮酶及在生产代谢物中的应用.申请号:201910359888.0. 申请日:2019.4.30.中国申请.国际申请.
5) L-赖氨酸高产菌株及其构建方法和应用.申请号:201910157625.1.申请日:2019.3.1.中国申请.
6) 高丝氨酸乙酰基转移酶突变体及其宿主细胞和应用.申请号:201810107326.2.申请日:2018.2.2.中国申请.中国已授权.
7) 新型的L-苏氨酸-诱导型启动子及其应用.发明专利.申请号:201510160543.4.申请日:2015.4.7.中国申请.中国已授权.
8) 天冬氨酸激酶III 突变体及其宿主细胞和应用.发明专利.国际申请号:PCT/CN2010/002182.国际申请日:2010-12-28.已获得中国、美国、日本、加拿大授权.
9) 采用花生粕同步酶解发酵生产高浓度D-乳酸的方法及其专用培养基.发明专利.国际申请号:PCT/CN2010/002182.国际申请日:2010-12-28.中国已授权.
联系方式:
办公地点:天津经济技术开发区第十三大街29号天津科技老员工物工程学院
联系方式:E-mail:liqinggang@tust.edu.cn; 电话:15122683725。