王洪彬,博士,天津科技老员工物工程学院教授,博士生导师。现任微生物教研室主任,国家自然科学基金委评审专家、中国发酵工业协会酶制剂分会会员、中国食品行业智库专家、天津微生物学会会员、天津食品学会会员。
主讲课程:
本科生:微生物学实验
研究生:生物与医药仪器分析
科研方向:
[1] 酶工程和发酵工程, 以蛋白酶的分子改造、发酵、分离和应用为主。
[2] 生物合成和绿色制造。
[3] 肠道微生态与疾病和健康的关系。
[4] 蛋白质组学和代谢组学等系统生物学分析技术及其应用。
科研项目:
近年来先后承担国家自然科学基金项目2项,国家重大研发计划项目子项目4项以及天津市科技支撑项目等多项省部级项目。
[1] 国家重点研发计划项目:人工载体多酶组装技术在重要生物化学品合成中的应用,2021-2023/12
[2] 国家重点研发计划项目:医药与食品工业酶高效制备与催化技术创新,2021-2024.
[3] 国家自然科学基金面上项目:基于末端组学深度解析食品碱性蛋白酶的动态水解规律及其位点选择特性,2019-2022。
[4] 国家重点研发计划项目:益生菌健康功能与基于肠道微生物组学的食品营养代谢机理研究,2017-2020。
[5] 国家自然科学基金青年项目:可控酶解制备寡肽过程中肽谱变化规律的研究,2014-2016
[6] 国家自然科学基金国际(地区)合作与交流项目:丝状真菌来源耐热脂肪酶的挖掘及其酶学特性的研究,2014-2017
科研成果:
发表SCI论文40多篇,发明专利14项。近几年以第一作者或通讯作者发表学术论文如下:
[1] Metabolomic profiling for the preventive effects of dietary grape pomace against colorectal cancer. J Nutr Biochem. 2023, 116:109308.
[2] Crosslinking Mechanism on a Novel Bacillus cereus Transglutaminase-Mediated Conjugation of Food Proteins. Foods. 2022; 11(22):3722
[3] Insights into the mechanism on the high-temperature activity of transglutaminase from Bacillus clausii and its crosslinked mode at protein level, Biochemical Engineering Journal, 2022, 185: 108544
[4] Targeted profiling of rodent unconjugated bile acids using GC-MS to determine the influence of high-fat diet. Biomedical chromatography: BMC. 2022; 36(9):e5428.
[5] Metabolomic Insights Into the Preventive Effects of Grape Pomace Against Colorectal Cancer. Curr Dev Nutr. 2021, 5(2):612
[6] Carboxypeptidase Y Assisted Disulfide-Bond Identification with Linearized Database Search, Analytical Chemistry. 2021, 93(45): 14940–14945
[7] Evaluation and verification of the characteristic peptides for detection of Staphylococcus aureus in food by targeted LC-MS/MS, Talanta, 2021: 122794.
[8] Insight into the cross-linking preferences and characteristics of the transglutaminase from Bacillus subtilis by in vitro RNA display, LWT,2021,151:112152.
[9] Screening of the candidate inhibitory peptides of subtilisin by in vitro RNA display technique, International Journal of Biological Macromolecules, 2020, 163:1162-1167
[10] Evaluation of the site-unspecified peptide identification method for proteolytic peptide mapping. RSC Adv., 2020,10, 37182-37186.
[11] Characterization and application of a novel laccase derived from Bacillus amyloliquefaciens. International journal of biological macromolecules, 2020, 150:982-990
[12] Targeted Analysis of Candidate Characteristic Peptides of staphylococcus aureus[J]. Chin.J. Anal. Chem.,2020, 48(7) :921-927.
[13] A metabolomic explanation on beneficial effects of dietary Goji on intestine inflammation, Journal of Functional Foods, 2019, 53:109-114.
[14] A sensitive GC/MS detection method for analyzing microbial metabolites short chain fatty acids in fecal and serum samples, Talanta, 2019, 196:249-254.
[15] A comparative proteomics method for multiple samples based on a 18O-reference strategy and a quantitation and identification-decoupled strategy, Talanta, 2017, 171:166-172.
[16] A sample pretreatment method to suit the metabolomics analysis of Bacillus licheniformis based on GC-MS. Analytical Methods, 2017, 9, 2299-2304
[17] Electro-ultrafiltration to remove sodium dodecyl sulfate in proteins extracted for proteomics. RSC Advances, 2017, 7, 25144-25148
[18] Heat acclimation of Bifidobacterium longum and proteomic changes behind it. Probiotics and Antimicrobial Proteins, 2017, 9(3): 255-261.
代表性发明专利:
[1] 一种氢化可的松生物催化转化率的快速检测方法,ZL201511014228.7,2019。
[2] 一种布氏乳杆菌发酵-转化-分离偶联制备甘露醇的方法,ZL201610851713.8,2019。
[3] 一种抗自切和高比活力胰蛋白酶突变体. ZL202010414080.0, 2022。
[4] 一种检测阪崎肠杆菌的新方法、应用及检测试剂盒。ZL202110991715.8. 2021。
[5] 谷氨酰胺转氨酶交联肽的筛选,ZL202010008938.3,2022。
[6] 一种芽孢杆菌来源碱性蛋白酶的新型抑制肽,ZL202110191324.8,2022。
教学成果和指导员工获奖:
[1] 2019年天津市工程专业学位优秀教学成果,省部级一等奖
[2] 第三届天津市高校教师教学创新大赛,省级三等奖;
[3] 参编规划教材《微生物学实验技术》和《酶工程原理与技术》
[4] 主讲课程《微生物学实验》,获批校级线上线下混合建设一流课程;
[5] 劳动课程《微世界艺术创作》,获批校级劳动教育精品课程;
[6] 指导员工参加2021全国老员工生命科学竞赛,获全国一等奖;
[7] 指导员工参加第二届全国老员工生命科学创新创业大赛,获全国一等奖
[8] 指导员工参加全国老员工生命科学竞赛(2021,科学探究类),省级三等奖
[9] 指导员工参加第五届微生物培养皿艺术大赛,获全国金奖;
[10] 指导员工参加第四届微生物培养皿艺术大赛,获全国银奖;
[11] 指导员工参加第三届微生物培养皿艺术大赛,获全国金奖;
[12] 指导员工参加“老员工创新创业训练计划”项目,市级
[13] 指导员工参加2019年度“中饮协-康师傅&战马杯”天津市老员工饮料创新大赛,省级一等奖
[14] 指导员工参加第八届老员工科技创新作品与专利成果展示推介会,获全国三等奖
[15] 指导员工参加2021年度“中饮协-康师傅&战马杯”天津市老员工饮料创新大赛,市级三等奖
联系方式:
办公地点:天津经济技术开发区第十三大街29号天津科技老员工物工程学院
邮政编码:300457
Email: whb@tust.edu.cn